IN VITRO AND IN VIVO FORMULATION AND EVALUATION OF ANTI-AGING CREAM WITH PROPOLIS EXTRACT

Abstract

Propolis contains flavonoids with antioxidant activity that can prevent the impact of UV exposure. Propolis has antioxidant activity, so its components can be used as an anti-aging (AA) cream cosmetic formulation. This development can be used as innovations in propolis products. AA cream is usually made of fatty acids and their derivatives. This study aims to formulate an AA cream preparation with propolis extract and its concentration variations to obtain a good physico-chemical formulation. The AA cream preparations with propolis extract use variations in concentration with a difference of 5%, 10%, and 15%. The physical and chemical evaluations included organoleptic tests, homogeneity, spreadability, adhesion, emulsion type, viscosity, and pH tests. The antioxidant value used the DPPH method. The statistical test used SPSS One-Way ANOVA, followed by a dependent test with a confidence level of 95%. As a result, the selected formula was Formula-1 with a 5% propolis concentration. The potential IC₅₀ of the three formulas based on the antioxidant test before the cycling test were: F1 = 5.16 ppm, F2 = 71.47 ppm, F3 = 159.93 ppm; while after the cycling test were: F1 = 29.55 ppm, F2 = 117.9 ppm, and F3 = 228.61 ppm.